Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Danio rerio) |
oep (tdgf1) | ZFIN | ZDB-GENE-990415–198 | |
| Gene (Danio rerio) |
ndr1 (sqt) | ZFIN | ZDB-GENE-990415–256 | |
| Gene (Danio rerio) |
ndr2 (cyc) | ZFIN | ZDB-GENE-990415–181 | |
| Gene (Danio rerio) |
smad2 | ZFIN | ZDB-GENE-990603–7 | |
| Gene (Danio rerio) |
vg1 (gdf3) | ZFIN | ZDB-GENE-980526–389 | |
| Gene (Danio rerio) |
lft1 | ZFIN | ZDB-GENE-990630–10 | |
| Gene (Danio rerio) |
lft2 | ZFIN | ZDB-GENE-990630–11 | |
| Strain, strain background (Danio rerio) | AB | ZIRC | ZDB-GENO-960809–7 | |
| Strain, strain background (Danio rerio) | TL | ZIRC | ZDB-GENO-990623–2 | |
| Genetic reagent (Danio rerio) | oeptz57 | Hammerschmidt et al., 1996 | RRID:ZDB-ALT-980203-1256 | |
| Genetic reagent (Danio rerio) | sqttcz35 | Feldman et al., 1998 | RRID:ZDB-ALT-000913-2 | |
| Genetic reagent (Danio rerio) | cycm294 | Sampath et al., 1998 | RRID:ZDB-ALT-980203-609 | |
| Genetic reagent (Danio rerio) | smad2vu99 | Dubrulle et al., 2015 | RRID:ZDB-ALT-150807-1 | |
| Genetic reagent (Danio rerio) | vg1a165 | Montague and Schier, 2017 | RRID:ZDB-ALT-180515-7 | |
| Genetic reagent (Danio rerio) | lft1a145 | Rogers et al., 2017 | RRID:ZDB-ALT-180417-4 | |
| Genetic reagent (Danio rerio) | lft2a146 | Rogers et al., 2017 | RRID:ZDB-ALT-180417-5 | |
| Recombinant DNA reagent | pJZoepFlag1-2 | Zhang et al., 1998 | Template for in vitro transcription | |
| Recombinant DNA reagent | SV40NLS-sfgfp in pCS2+ | Gift from Dr. Jeffrey Farrell | Template for in vitro transcription | |
| Recombinant DNA reagent | sqt-sfGFP in pCS2+ | Montague and Schier, 2017 | Template for in vitro transcription | |
| Recombinant DNA reagent | vg1-halotag in pCS2+ | This study | Plasmid can be obtained by reaching out to N.L. | Template for in vitro transcription. |
| Recombinant DNA reagent | sqt in pCS2+ | Müller et al., 2012 | Template for in vitro transcription | |
| Antibody | Anti-phospho-smad2/3 (rabbit monoclonal) |
Cell Signaling Technology | #18338 | 1:1000 dilution |
| Antibody | Anti-GFP (chicken monoclonal) |
Aves Labs | (RRID:AB_2307313) | 1:1000 dilution |
| Antibody | Anti-eCdh1 (mouse monoclonal) |
BD Biosciences | #610181 (RRID:AB_397580) |
1:100 dilution |
| Antibody | Goat α-rabbit Alexa 647 conjugate (goat monoclonal) |
Thermo-Fisher Scientific | A-21245 (RRID:AB_2535813) |
1:2000 dilution |
| Antibody | Goat α-chicken Alexa 488 (goat monoclonal) |
Thermo-Fisher Scientific | A-11039 (RRID:AB_142924) |
1:2000 dilution |
| Antibody | Goat α -mouse IgG (H + L)-Alexa 488 (goat monoclonal) |
Thermo-Fisher | A-32723 (RRID:AB_2633275) |
1:750 dilution |
| Peptide, recombinant protein | Pronase | Millipore Sigma | 53702 | |
| Commercial assay or kit | mMessage mMachine Sp6 kit | Thermo-Fisher | AM1340 | |
| Commercial assay or kit | E.Z.N.A. Cycle Pure | Omega Bio-Tek | D6492-01 | |
| Commercial assay or kit | E.Z.N.A. Total RNA Kit I | Omega Bio-Tek | R6834-01 | |
| Chemical compound, drug | kDa Alexa488-dextran conjugate | Thermo-Fisher | D34682 | |
| Chemical compound, drug | Janelia Fluor HaloTag Ligand 646 | Promega | GA1120 | |
| Software, algorithm | ImageJ/FIJI | ImageJ/FIJI | RRID:SCR_002285 | Image Analysis |
| Software, algorithm | MATLAB | Mathworks | RRID:SCR_001622 | Image Analysis, Simulations |