Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Recombinant DNA reagent | pENN.AAV5.CAMKII.GCaMP6f.WPRE.SV40 | Addgene | Cat: 100834-AAV5 RRID:Addgene_100834 |
Lot # v59618 |
| Recombinant DNA reagent | rAAV5-CAMKIIa-eArchT3.0-eYFP | UNC-CH vector core | Deisseroth | Lot # V4883D |
| Recombinant DNA reagent | rAAV5-CAMKIIa-eYFP | UNC-CH vector core | Deisseroth | Lot # AV4808I |
| Recombinant DNA reagent | pAAV8-hSyn-hM4D(Gi)-mCherry | Addgene | Cat: 50475-AAV8 RRID:Addgene_50475 |
Lot # v5483 |
| Recombinant DNA reagent | pAAV8-hSyn-mCherry | Addgene | Cat: 114472-AAV8 RRID:Addgene_114472 |
|
| Other | Optical fiber (photometry) | Neurophotometrics | Diameter: 200 µm; NA: 0.37; Length: 8–8.5 mm | |
| Other | Optical fiber (manipulation) | Thorlabs | Cat: FT200UMT | Core: 200 µm; NA: 0.39; Length: 8–8.5 mm |
| Other | Optical ferrules | Kientec | Cat: FAZI-LC-230 | |
| Other | Guide cannula | Plastics One | Cat: C313G/SPC | Length: cut to 4 mm below pedestal |
| Chemical compound, drug | Clozapine N-oxide | Tocris | Cat: 4936/10 CAS: 34233-69-7 |
|
| Other | Dustless precision Chocolate-flavored purified pellets | Bio-Serv | Cat: F0299 | 45 mg |
| Other | Sucrose | Ralphs | UPC: 0001111083805 | |
| Antibody | Chicken polyclonal anti-GFP antibody | Abcam | Cat: ab13970 | (1:1000) |
| Antibody | Goat polyclonal anti-chicken IgG, Alexa Fluor 488 conjugate | Abcam | Cat: ab150169 RRID:AB_2636803 |
(1:500) |
| Antibody | Rabbit polyclonal anti-DsRed antibody | Takara Bio | Cat: 632496 RRID:AB_10013483 |
(1:1000) |
| Antibody | Goat polyclonal anti-rabbit IgG, Alexa Fluor 594 conjugate | Invitrogen | Cat: A-11012 RRID:AB_253407 |
(1:500) |
| Other | ProLong Gold Antifade Mountant with DAPI | Invitrogen | Cat: P36931 | |
| Chemical compound, drug | Paraformaldehyde | Sigma | Cat: P6148 | |
| Software, algorithm | MED-PC IV | Med Associates, Inc | RRID:SCR_012156 | |
| Software, algorithm | GraphPad Prism | GraphPad Software | RRID:SCR_002798 | Version: 8 |
| Software, algorithm | MatLab | MathWorks | RRID:SCR_001622 | Version: 2019a |
| Software, algorithm | SPSS | IBM | RRID:SCR_019096 | Version: 26 |
| Software, algorithm | Bonsai | Bonsai | RRID:SCR_017218 | Version: 2.3 |
| Software, algorithm | Minianalysis | Synaptosoft | RRID:SCR_002184 | Version 6 |
| Software, algorithm | BZ-X Analyze software | Keyence | RRID:SCR_017205 | |
| Software, algorithm | Zeiss Zen Blue software | Zeiss | RRID:SCR_013672 | |
| Software, algorithm | Illustrator | Adobe | RRID:SCR_010279 | |
| Software, algorithm | ImageJ | NIH | RRID:SCR_003070 | |
| Software, algorithm | Excel | Microsoft | RRID:SCR_016137 |