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. 2021 Jun 16;10:e66954. doi: 10.7554/eLife.66954

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© 2021, Koiwai et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 9. — (A) Differential interference contrast (DIC) image of unsorted total hemocytes. (B) Dye-stained total hemocytes. (C) DIC imaging and dye staining of region 1 (R1)-sorted hemocytes. (D) DIC imaging and dye staining of region 2 (R2)-sorted hemocytes. (E) Fluorescence-activated cell sorting (FACS) analysis of hemocytes. Based on the forward scatter (FSC) and side scatter (SSC) two-dimensional space, two regions (R1 and R2) were obtained. (F) Differential gene expression analysis between R1 and R2 of hemocytes sorted using FACS. ∆∆Ct values were analyzed using qRT-PCR. Higher ∆∆Ct values indicate a higher accumulation of mRNA transcripts. The p values shown in the figures are represented by *p<0.05.

Figure 9—source data 1. Source data for CT values of each gene used for Figure 9F.

elife-66954-fig9-data1.xlsx^{(13.9KB, xlsx)}

Figure 9—figure supplement 1. — (A) Differential interference contrast (DIC) image of unsorted total hemocytes. (B) Dye-stained total hemocytes. (C) DIC imaging and dye staining of region 1 (R1)-sorted hemocytes. (D) DIC imaging and dye staining of region 2 (R2)-sorted hemocytes. (E) Fluorescence-activated cell sorting (FACS) analysis of hemocytes. Based on the forward scatter (FSC) and side scatter (SSC) two-dimensional space, two regions (R1 and R2) were obtained. (F) Differential gene expression analysis between R1 and R2 of hemocytes sorted using FACS. ∆∆Ct values were analyzed using qRT-PCR. Higher ∆∆Ct values indicate a higher accumulation of mRNA transcripts. The p values shown in the figures are represented by *p<0.05.

Figure 9—source data 1. Source data for CT values of each gene used for Figure 9F.

elife-66954-fig9-data1.xlsx^{(13.9KB, xlsx)}