Skip to main content
. 2021 Jun 24;9:693079. doi: 10.3389/fcell.2021.693079

FIGURE 5.

FIGURE 5

SENP +/− mice were less likely to suffer severe GIOP probably with undisturbed osteogenesis by Dex-induced ROS upregulation. (A) Represented images of three-dimensional (3D) cancellous bone restoration (a–h) of the distal femur cancellous bone in 8–12 week-old WT and SENP3 +/− mice with saline (n = 5, a,e), NAC alone (n = 5, b,f), Dex alone (n = 5, c,g) and Dex + NAC (n = 5, d,h), and three-dimensional (3D) cortical bone restoration (i–p) of the distal femur cortical bone in 8–12-week-old WT and SENP3 +/− mice with saline (n = 5, a,e), NAC alone (n = 5, b,f), Dex alone (n = 5, c,g) and Dex + NAC (n = 5, d,h); (B) Trabecular bone volume/tissue volume ratios (%) (BV/TV, a), trabecular thickness (Tb.Th, b) and cortical thickness (Ct.Th, c) the distal femur cancellous and cortical bone in 8–12-week-old WT and SENP3 +/− mice with saline (n = 5), NAC alone (n = 5), Dex alone (n = 5), and Dex + NAC (n = 5). (C) HE staining (a–h) of distal femur on paraffin-embedded bone sections in in 8–12-week-old WT and SENP3 +/− mice with saline, NAC alone, Dex alone and Dex + NAC (scale bar, 100 mm; red arrow heads point to osteoblasts); osteoblast surface/bone surface ratios (Ob.S/BS) (i) is shown on the right. Data are shown as the mean ± SEM. *p < 0.05, **p < 0.01, ns: not significant. All the data were obtained from at least three independent experiments.