Figure 3.

Curcumin synergistically enhances the antitumor proliferation effect of sestrin2. The changes in the proliferation ability of the PANC-1-sestrin2 overexpression group (sestrin2+) and sestrin2+/curcumin group (a); the cloning counts are presented in the form of a histogram (b). The changes in the proliferation ability of the CFPAC-1-sestrin2 overexpression group (sestrin2+) and sestrin2+/curcumin group (c); the cloning counts are presented in the form of a histogram (d); data are represented as the mean ± SEM (n = 3). ^∗∗P < 0.005 compared with control groups, ^∗∗∗∗P < 0.0001 compared with control groups, and ^##P < 0.005 compared with sestrin2+ group; the CCK-8 assay was utilized to observe the change in the proliferation ability between the sestrin2+ group, sestrin2+/curcumin group, control group, and blank group in PANC-1 and CFPAC-1 cells (e, f).