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. 2021 Jul 1;2021:9995342. doi: 10.1155/2021/9995342

Figure 3.

Figure 3

The cardioprotective effects of Wog involved in activating Nrf-2-mediated antioxidant responses. (a) Western blot analysis of Nf-2 following siRNA transfection in H9C2 cells, where GAPDH was used as the loading control (n = 3); (b, c) the mRNA expression of Ho-1 and Nqo-1 by RT-qPCR (n = 4); (d, e) representative images of DCF staining from H9C2 cells (magnification: ×200, scale bar: 20 μm); (f, g) representative images of rhodamine-labeled phalloidin staining from H9C2 cells (magnification: ×200, scale bar: 20 μm). ∗P < 0.05, ∗∗P < 0.01, and ∗∗∗P < 0.001 compared to Ctrl; #P < 0.05, ##P < 0.01, and ###P < 0.001 compared to AngII; &P < 0.05, &&P < 0.01, and &&&P < 0.001 compared to AngII + Wog.