Figure 5.

The cardioprotective effects of Wog involved in the modulation the Nrf-2-mediated antioxidant responses in vivo. (a–c) mRNA levels of Nrf-2 target genes Ho-1 and Nqo-1 as determined by qRT-PCR (n = 5); (d, e) immunohistochemical staining of heart tissues for Keap-1, the positive region with the red arrow; (f, g) heart lysates were probed for Keap-1 levels with Western blotting. GAPDH was used as the loading control (n = 3); (h) schematic diagram summarizes the molecular mechanisms by which Wog ameliorates cardiac hypertrophy by inhibiting oxidative stress. Wog as a promising natural agent, which protects against cardiac hypertrophy by activating the Nrf-2-mediated antioxidant responses. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001 compared to sham; ^#P < 0.05, ^##P < 0.01, and ^###P < 0.001 compared to TAC.