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. 2021 Jul 1;2021:6668463. doi: 10.1155/2021/6668463

Figure 3.

Figure 3

Pellino3 knockout causes decreased cytokine secretion and increased virus multiplication. (a) Total RNA was isolated from WT and Peli3−/− BMDMs and converted to first-strand cDNA. This was used as a template for conventional PCR amplifying genes as indicated. Products were resolved as described under Materials and Methods. (b) Wild-type and Peli3−/− BMDMs were infected with VSV at MOI 1.5. Cell culture supernatants were collected 16 h of postinfection. Concentration of type I IFN was measured by bioassay; level of IP-10 and TNF was measured by ELISA as described under Materials and Methods. (c) Wild-type and Peli3−/− BMDM cells were infected with VSV at MOI 0.5. Cell supernatants were collected 24 h of postinfection to determine viral titer as described under Materials and Methods. Results are representative of at least three independent experiments performed in triplicate (mean ± S.E.). Statistical analysis was carried out using the unpaired Student t-test using GraphPad Prism 7.04. p values of less than or equal to 0.05 were considered to indicate a statistically significant difference (∗p ≤ 0.05, ∗∗p ≤ 0.01, and ∗∗∗p ≤ 0.001).