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. 2021 Jun 14;49(12):7011–7034. doi: 10.1093/nar/gkab461

Figure 4.

Figure 4.

(A) Growth curves of HEK293T CTRL (blue) and ADAT2 KD (red) cells showing total living cells over time. Y-axis is set in logarithmic scale and an exponential trendline was fit to the data points. Data points correspond to the mean and SD of three biological replicates. ***: P-value < 0.001 (t-test). See also Supplementary Figure S3A–C. (B) Cell cycle analysis of HEK293T CTRL (blue-bordered bars) and ADAT2 KD (red-bordered bars) cells as evaluated by FACS over a 12-h period. Shown are the mean and SD of biological duplicates. Statistical significance is depicted only for percentage of cells in G1 phase at every time point. n.s.: not statistically significant. *: P-value < 0.05. **: P-value < 0.01 (t-test). (C) Upper panel: ADAT2 protein levels on the indicated cell lines. GAPDH is used as gel loading control. Quantification of ADAT2 bands have been normalized to GAPDH and relative to HEK293T CTRL cells. Lower panel: Growth curves as in (A) of HEK293T CTRL cells (black) and of HEK293T CTRL and ADAT2 KD cells upon stable re-expression of ADAT2 (‘pLenti-hADAT2′) (blue and red, respectively). n.s.: not statistically significant (t-test). (D) Representative light microscopy images of trypsin-treated HEK293T CTRL and ADAT2 KD cells at 24 (upper panels) and 48 (lower panels) h after plating. See also Supplementary Figure S3D.