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. 2021 Jun 14;49(12):7053–7074. doi: 10.1093/nar/gkab494

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Nop53 depletion differentially affects the association of the nucleocytoplasmic AFs Arx1 and Nmd3 with pre-60S intermediates. (A) Schematic representation of the pre-rRNA processing in S. cerevisiae, highlighting the stages during which Nop53 is associated with the pre-60S and the hybridization position of the Northern blot probes. (B) The pre-rRNAs copurified (IP) with Arx1-TAP and Nmd3-TAP in biological triplicates both in Nop53 depleted (−) and non-depleted (+) conditions were assayed along with the Input (total RNA) by Northern blot using the indicated probes. The empty TAP tag was used as a negative control and the Δrrp6 strain as a control for the accumulation of 5.8S+30 pre-rRNA. *non-specific band.