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. 2021 Jun 14;49(12):7053–7074. doi: 10.1093/nar/gkab494

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — Yvh1 recruitment to the pre-60S is impaired upon depletion of Nop53. (A) Northern blot analysis of the pre-rRNAs affinity-purified (IP) in biological triplicates with Yvh1-TAP and Lsg1-TAP both in Nop53 depleted (−) and non-depleted (+) conditions, together with the Input (total RNA), using the indicated probes. The Δrrp6 strain is a control for the accumulation of 5.8S+30 pre-rRNA. *non-specific band. (B, C) Glycerol density gradient sedimentation of the TAP-tagged AFs (B) Yvh1 and (C) Lsg1 in Nop53 depleted (−) and non-depleted (+) conditions. Whole-cell lysates were loaded on a 10–30% linear glycerol gradient. The 24 fractions collected were analyzed by western blot with anti-CBP, anti-GFP and anti-uL18. ** indicates the Lsg1-TAP band close to GFP-Nop53 (In: input - whole cell lysate; M: protein MW marker).