Figure 7.
nop53 N-terminal truncated mutants impair the recruitment of Yvh1 to the pre-60S. Sedimentation profile analysis of Yvh1-TAP expressed in the conditional strain Δnop53 carrying (A) the positive control GFP-NOP53FL full length (+), (B) the negative control empty vector (−), or the GFP-tagged N-terminal truncated mutants (C) nop53Δ1–26, (D) nop53Δ1–47, (E) nop53Δ48–98 and (F) nop53Δ1–71. Whole-cell lysates were subjected to 10–30% glycerol density gradient sedimentation followed by top to bottom fractionation. All the odd fractions collected were analyzed by western blot with anti-CBP, anti-GFP, and anti-uL18. (In: input – whole cell lysate; M: protein MW marker).