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. 2021 Jun 14;49(12):6880–6892. doi: 10.1093/nar/gkab495

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Effects of ABK on the release factor mediated peptide release. (A) BOP-fluorescence traces for the release of BOP-Met-Phe-Leu tripeptide from the ribosome by RF2 (1 μM) at indicated ABK concentrations. Solid lines are double exponential fits of the data. (B) Decrease in the rates of peptide release by RF2 (1 μM) with increasing concentrations of ABK. The rates are estimated from the predominant fast phase of the BOP-fluorescence traces. (C) BOP-fluorescence traces for the release of BOP-Met-Phe-Leu tripeptide from the ABK (10 μM) saturated ribosomes (0.1 μM) at indicated concentrations of RF2. (D) Hyperbolic plot showing increase in the rate of peptide release with increasing RF2 concentrations. The values of k_cat and K_M were estimated from the double reciprocal plot (inset). Error bars are SEM of the data obtained from at least two independent experiments.