Table 1.

Calculation of the average distance travelled by rightward replication forks emanating from the IZ located in 5′ of DMD^Tet. The 5′ IZ was considered to be fully efficient. Replication fork velocity was assumed to be constant throughout S-phase. fS1, fS2, fS3 and fS4 replication timing values correspond to the percentage of BrdU-labelled DNA relative to total S-phase in S1, S2, S3 and S4 fractions, respectively, at the position of the 5′ IZ (data from Figure 1F in (46); average percentage from two independent experiments). vRF, velocity of rightward forks

Parameter		Value	Comments
Doubling time		552 min	Data from Supplementary Figure 1G in ref. (46)
% of cells in S phase		69.4	Data from Supplementary Figure 1H in ref. (46)
Estimated S-phase duration		383 min	= % of cells in S phase*doubling time
Median velocity of rightward forks emanating from the IZ located in 5′ of DMDTet		2.1 kb/min	vRF; see Supplementary Figure S1B
Theoretical distance travelled by rightward forks emanating from the IZ located in 5′ of DMDTet starting in	S1	804 kb	dS1 = 1*S-phase duration*vRF
	S2	603 kb	dS2 = 0.75*S-phase duration*vRF
	S3	402 kb	dS3 = 0.5*S-phase duration*vRF
	S4	201 kb	dS4 = 0.25*S-phase duration*vRF
% of rightward forks emanating from the IZ located in 5′ of DMDTet starting in	S1	2.2	fS1; data from Figure 1F in ref. (46)
	S2	6.2	fS2; data from Figure 1F in ref. (46)
	S3	26.2	fS3; data from Figure 1F in ref. (46)
	S4	65.4	fS4; data from Figure 1F in ref. (46)
Average distance travelled by rightward forks emanating from the IZ located in 5′ of DMDTet		292 kb	= dS1*fS1+dS2*fS2+dS3*fS3+dS4*fS4