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. 2021 Jun 15;40(27):4604–4614. doi: 10.1038/s41388-021-01883-6

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A–B qRT-PCR showed the inhibitory effect of si-PTOV1-AS1 on EMT in MDA-MB-231 cells treated with TGF-β1. n = 4. C The inhibitory effects of si-PTOV1-AS1 on TGF-β1-induced EMT were detected by Western blot. n = 4. D Immunofluorescence staining of ZO-1 revealed the inhibitory effect of si-PTOV1-AS1 on the EMT process in MDA-MB-231 cells. n = 4. Scale bar, 20 μm. Wound healing assay (E) and Transwell assay (F) showed that silencing PTOV1-AS1 attenuated TGF-β1-induced cell migration and invasion. n = 6. Scale bar, 200 μm in (E) and 100 μm in (F). *P < 0.05; **P < 0.01. lncRNA long non-coding RNAs, EMT epithelial mesenchymal transition, qRT-PCR real-time quantitative reverse transcription PCR.