KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-DsRed/mCherry/tdTomato rabbit | Takara | Cat#632496; RRID: AB_10013483 |
| Anti-GFP chicken | Abcam | Cat#13970; RRID: AB_300798 |
| Goat anti-Chicken IgY (H+L) Secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat#A-11039, RRID: AB_2534096 |
| Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 555 | Thermo Fisher Scientific | Cat#A-21428; RRID: AB_2535849 |
| Bacterial and Virus Strains | ||
| pAAV1-ef1a-DIO-ChR2-EYFP | Gift from Karl Deisseroth | Addgene viral prep 20298-AAV1; RRID:Addgene_20297 |
| pAAV8-hSyn-DIO-hM3D(Gq)-mCherry | Krashes et al., 2011 | Addgene viral prep 44361-AAV8 RRID:Addgene_44361 |
| pAAV8-hSyn-DIO-hM4D(Gi)-mCherry | Krashes et al., 2011 | Addgene viral prep 44362-AAV8; RRID:Addgene_44362 |
| AAV9-Ef1a-DO-hChR2(H134R)-mCherry | Gift from Bernardo Sabatini (Kozorovitskiy et al, 2012) packaged by Vigene Biosciences |
Addgene plasmid # 37082; RRID:Addgene_37082 |
| AAV8-hSyn-DIO-mCherry | Krashes et al., 2011 | Addgene viral prep 50459-AAV8 RRID:Addgene_50459 |
| AAV8-Ef1a-DIO-synaptophysin-mCherry | Virovek | N/A |
| AAV-DJ-CMV-DIO-eGFP-2A-TeNT | Similar to (Campos et al., 2018) | Stanford Viral Core GVVCAAV-71 |
| AAV5-EF1a-DIO-EYFP-WPRE-pA | gift from Karl Deisseroth | Addgene viral prep 27056-AAV5; RRID:Addgene_27056 |
| AAV8-Ef1a-FLEX-TVA-mCherry | UNC vector core | N/A |
| EnvA-G-Deleted-Rabies-Egfp | Gene Transfer, Targeting and Therapeutics Core at Salk Institute | Cat#32635 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| CNO | Sigma | Cat#C0832-5MG |
| Saline | Medline | Cat#DYND1000 |
| MK-5046 | MedChemExpress | Cat#HY-14342 |
| LPS | Sigma | Cat#L6511-25mg |
| TTX | Abcam | Cat#ab120054 |
| 4-aminopyridine (4-AP) | Sigma | Cat#A7840 |
| DMSO | Sigma | Cat#472301-100ML |
| tamoxifen | Sigma | Cat#T5648-5G |
| Propranolol | Sigma | Cat#P0884-5G |
| Corn oil | Sigma | Cat#C8267-500ML |
| Experimental Models: Organisms/Strains | ||
| BRS3-Cre; B6.129S6(C3)-Brs3tm3.1(cre/ERT2)Rei/J | The Jackson Laboratory | Cat#032614 |
| Ai14 (Cre-dependent tdTomato); B6.Cg-Gt(ROSA)26Sortm14(CAG-tdTomato)Hze/J | The Jackson Laboratory | Cat#007914 |
| Ai6 (Cre-dependent GFP); B6.Cg-Gt(ROSA)26Sortm6(CAG-ZsGreen1)Hze/J | The Jackson Laboratory | Cat#007906 |
| Gad2-2a-NLS-mCherry (Gad2-mCherry); B6;129S-Gad2tm1.1Ksvo/J | The Jackson Laboratory | Cat#023140 |
| C57BL/6J | The Jackson Laboratory | Cat#000664 |
| Software and Algorithms | ||
| Prism v9.0.2 | Graphpad | www.graphpad.com |
| VitalView v5.0 | Starr Life Sciences | www.starrlifesciences.com/ |
| Zen (Black) | Zeiss | https://www.zeiss.com/microscopy/us/home.html |
| Ponemah v6.30 | Data Sciences International | https://www.datasci.com/ |
| EthoVision XT | Noldus | https://www.noldus.com/ |
| Other | ||
| E-mitter (body temperature telemetry system – Implants)Starr Life Sciences, Oakmont, PA, USA | Starr Life Sciences | Cat# G2 E-Mitter |
| E-mitter receiver/energizer (body temperature telemetry system) | Starr Life Sciences | Cat# ER4000 |
| Intra-arterial pressure telemetry probe | Data Sciences International | HD-X10 or HD-X11 |
| PhysioTel RPC-1 receiver | Data Sciences International | RPC-1 |
| CLAMS | Columbus Instruments | Sn# 110117 |