Table 1.

Results of in vivo A. gambiae enhancer validations.

Predicted Enhancer	Drosophila melanogaster ortholog(s)a	Tested Coordinatesb	Training set	Expected expressionc	Observed expression	Match gene?d	Match training?e
C01-800	Inside CG7611	2L:25564979-25565526	mapping2.salivary_gland	Expressed ubiquitously in the embryo, high levels of expression in the larval salivary glands and midgut	clypeolabrum (st. 14); anterior pharynx (st. 15–16); midgut (starting st. 14); subset cells in amnioserosa; weak hindgut	U	N
C02-355	Inside of Atg5	3L:13487751-13488250	mapping1.salivary_gland	Pupal salivary glands	Salivary gland (possibly due to vector-driven expression)	U	U
C02-783	Downstream of β-Man	X:2179509-2180000	mapping1.salivary_gland	Salivary gland and midgut	Fat body, gut, salivary gland, other	Y	U
C02-221	Inside of Hnf4	2R:16555950-16556550	mapping1.endoderm and mapping1.salivary_gland	Embryonic salivary gland, fat body, gut, oenocyte	Subset of cells in stomatogastric nervous system; lateral segmentally repeated cells (probable oenocyte precursors)	Y	Y
C02-333	Exonic region of CG30460	3L:3138501-3139001	mapping1.endoderm	Midgut and muscle	Proventriculus	Y	Y
C02-188	Downstream of Ncc69 and upstream of CrzR	2R:6200001-6200501	mapping1.endoderm	Midgut, foregut, and late salivary gland	No expression	N	N
C01-333	Upstream of Stacl	2L:13256901-13257589	mapping1.pns	Embryonic peripheral and central nervous systems	Peripheral nervous system (chordotonal organs)	Y	Y
C01-4868	Inside of eyg	3L:24132710-24134004	mapping1.imaginal_disc also: mapping1.trachea, mapping2.neuronal	Embryonic nervous system expression	Central nervous system, brain, posterior, faint dorsal epidermis	Y	Y
C01-2915	Upstream of CG6154	2R:24703930-24704594	mapping1.ventral_ectoderm	Not well characterized	Tracheal system - different subset of cells then C01-4888	U	N
C01-4888	Inside of kn	3L:24700700-24701525	mapping1.somatic_muscle & mapping2.mesoderm also: neuroectoderm, mesectoderm	Imaginal tissue, sensory system, nervous system, digestive system	Tracheal system - different subset of cells then C01-2915	N	N
C01-2230	Inside of Desat1	2R:8869422-8870077	mapping0.dv_neurogenicectoderm	Ubiquitous, strong expression in midgut	No expression	N	N

^aBased on closest D. melanogaster gene; assignments as per FlyBase (Thurmond et al., 2019)

^bAnopheles gambiae PEST P4 coordinates

^cBased on FlyBase (Thurmond et al., 2019) gene report

^dObserved expression is broadly consistent with that of putative target gene. Y, yes; N, no; U, unable to determine (target gene expression is unknown or ubiquitous, or reporter gene expression is confounded with vector-only expression).

^eObserved expression is consistent with expectation based on training set. Y, yes; N, no; U, unable to determine