Extended Data Table 1 |.
Cryo-EM Data.
| MFSD2A_GG-2AG3 complex (EMD-23883) (PDB ID 7MJS) |
|
|---|---|
| Data collection and processing | |
| Magnification | 105,000 |
| Voltage (kV) | 300 |
| Electron exposure (e-/Å2) | 58.0 |
| Dose rate (e-/pixel/s) | 16 |
| Defocus range (μm) | 1.75 – 2.5 |
| Pixel size (Å) | 0.83 |
| Symmetry imposed | C1 |
| Initial micrographs collected (no.) | 15,516 |
| Final micrographs processed (no.) | 13,300 |
| Initial particle images (no.) | 5,049,988 |
| Final particle images (no.) | 175,738 |
| Map resolution (Å) | 3.03 |
| FSC threshold | 0.143 |
| Map sharpening B factor (Å2) | −65.6 |
| Residue range | 36–267 and 280–505 (MFSD2A) |
| 2–114 (2AG3 vFab light chain) | |
| 4–135 (2AG3 vFab heavy chain) | |
| Model composition | |
| Non-hydrogen atoms | 5554 |
| Protein residues | 703 |
| Ligands | 4 |
| B factors (Å2) | |
| Protein | 57.65 |
| Ligand | 60.21 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.005 |
| Bond angles (°) | 1.010 |
| Validation | |
| MolProbity score | 1.79 |
| Clashscore | 8.62 |
| Rotamer outliers (%) | 0 |
| EM-Ringer Score | 3.52 |
| Ramachandran plot | |
| Favored (%) | 95.25 |
| Allowed (%) | 4.75 |
| Disallowed (%) | 0.00 |