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. 2021 Jul 8;12(7):686. doi: 10.1038/s41419-021-03957-5

Fig. 1. Autophagic flux is compromised in c-FLIP−/− cells.

Fig. 1

A WT and c-FLIP−/− MEFs were treated with torin 1 (Tor) (250 nM) and bafilomycin A1 (Baf) (100 nM) or cultured in a starvation medium (EBSS) and treated with bafilomycin A1 (Baf) (100 nM) for 3 h. Autophagic flux was assessed by measuring LC3 II and p62 protein levels by western blotting. β-Actin was used as a loading control. Data shown are representative of at least three individual experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 determined by two-way ANOVA. B WT and c-FLIP−/− MEFs were cultured in EBSS and treated with bafilomycin A1 for 3 h. Cells were fixed and stained with anti-LC3 antibody (green) and then analysed by confocal microscopy. The images and the graph shown are representative of at least three individual experiments. **p < 0.01, ***p < 0.001 determined by two-way ANOVA. Scale bar, 30 μm.