Fig. 3. WT and c-FLIP−/− MEFs were cultured in EBSS for increasing times (1, 2 and 3 h) and the expression levels of Beclin-1, Vps34 and P-Atg14 were analyzed.

A Beclin-1, B Vps34 and C P-Atg14 on Ser29 were studied by western blotting. β-Actin was used as a loading control. Data shown are representative of at least three individual experiments. *p < 0.05, **p < 0.01 determined by two-way ANOVA.