Skip to main content
. 2021 Jul 8;11:14088. doi: 10.1038/s41598-021-93623-9

Figure 4.

Figure 4

Bleomycin-induced pulmonary fibrosis is attenuated in Il11 knockout mice. (A) Schematic showing the induction of lung fibrosis in Il11−/− and wild-type mice. A single dose of bleomycin (BLM) was administered oropharyngeally and the mice were sacrificed 14 days post-BLM. (B) Representative gross lung anatomy of Il11−/− and wild-type mice 14 days post-BLM. (C) Masson’s trichrome staining of lung sections, (D) histology assessment of fibrosis and (E) total lung hydroxyproline content of Il11−/− and wild-type mice 14 days post-BLM. Scale bars: 100 µm. (Il11+/+ uninjured n = 3–4; Il11+/+  + BLM n = 7–10; Il11−/− uninjued n = 3–4; Il11−/− + BLM n = 7–10). Relative RNA expression of (F) Col1a1, (G) Col1a2, (H) Fn1, (I) Mmp2, (J) Timp1, (K) Il1b, (L) Il6 and (M) Ccl2 in lung lysates from Il11−/− and wild-type mice 14 days post-BLM (n = 4). Data shown as: centre line, median value; box edges, 25th and 75th percentiles; whiskers, minimum and maximum values. P values were determined by ANOVA (Tukey’s test).