Skip to main content
. 2021 Jul 8;7:176. doi: 10.1038/s41420-021-00524-x

Fig. 5. MiR-7641 regulated HIF-1α expression in breast cancer cells.

Fig. 5

A The target gene of miR-7641 was predicted using miRNA walk, Targetcan, and RNA22-HAS. B miR-7641 suppressed target gene expression in breast cancer cells. The expression of the possible target genes of miR-7641 was analyzed by real-time RT-PCR. C The predicted target sequence of HIF-1α and the mutated sequence of HIF-1α. D, E MiR-7641 suppressed the luciferase activity of HIF-1α in MDA-MB-231 and SKBR3 cells. Cells were transfected with miR-7641 mimics and plasmids with luciferase reporters and the luciferase activity was assayed by a dual-luciferase reporter system. F miR-7641 suppressed HIF-1α mRNA levels in breast cancer cells. Cells were transfected with miR-7641 mimics for 48 h, and total RNA was isolated for real-time RT-PCR analysis. G HIF-1α protein levels in breast cancer cells with miR-7641 overexpression. Cells were transfected with miR-7641 mimics for 48 h, and total protein was isolated for western blotting. *p < 0.05, **p < 0.01.