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. 2021 Jul 8;7:176. doi: 10.1038/s41420-021-00524-x

Fig. 6. MiR-7641 regulated cancer cell stemness and glycolysis via HIF-1α.

Fig. 6

A, B Cell proliferation analysis of SKBR3 and MDA-MB-231 cells. Cells were overexpressed HIF-1α and cultured with sEVs from NFs, CAFs, or CAFs with miR-7641 overexpression. Cell survival ability was assayed by the CCK8 kit. C, D Sphere formation analysis of spheres in SKBR3 and MDA-MB-231 cells. Cells were overexpressed HIF-1α and cultured with sEVs from NFs, CAFs, or CAFs with miR-7641 overexpression. The photos were taken under a microscope. E, F ECAR in MDA-MB-231 cells. Cells were overexpressed HIF-1α and cultured with sEVs from NFs, CAFs, or CAFs with miR-7641 overexpression, and ECAR was analyzed using seahorse. G, H ECAR in SKBR3 cells. Cells were overexpressed HIF-1α and cultured with sEVs from NFs, CAFs, or CAFs with miR-7641 overexpression, and ECAR was analyzed using seahorse. I, J The expression of stem cell and glycolysis markers in SKBR3 and MDA-MB-231 cells. Cells were overexpressed HIF-1α and cultured with sEVs from NFs, CAFs, or CAFs with miR-7641 overexpression. Stem cell and glycolysis-related genes were assayed by real-time RT-PCR. K Western blot analysis of the expression of cancer stemness and glycolysis associated proteins in MDA-MB-231 and SKBR3 cells. Cells were overexpressed HIF-1α and cultured with sEVs from NFs, CAFs, or CAFs with miR-7641 overexpression, and protein was isolated for western blotting. *p < 0.05, **p < 0.01.