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. 2021 Jul 8;11:14097. doi: 10.1038/s41598-021-93420-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of high glucose on H3K9 methylation. HRECs incubated in 5 mM (NG) or 20 mM (HG) D-glucose, or 20 mM L-glucose (L-Gl), for 96 h were analyzed for (a) H3K9me3 and (b) H3K9me2 at Rac1 promoter by ChIP technique using normal rabbit IgG (^) as an antibody control. Each measurement was made in duplicate 3–4 different cell preparation, and the values are represented as mean ± SD. *p < 0.05 vs NG.