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. 2021 Jul 8;11:14097. doi: 10.1038/s41598-021-93420-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Regulation of Rac1 gene transcripts and 5hmC at its promoter. Cells transfected with Suv39H1-siRNA (Suv-si) or Dnmt1-siRNA (Dnmt-si), incubated in high glucose for four days, were analyzed for Rac1 (a) gene transcripts by qRT-PCR using β-actin as a housekeeping gene and (b) 5hmC at its promoter by immunoprecipitating 5hmC in the genomic DNA , and quantifying by qRT-PCR using gene specific primers. Transfection efficiency of (c) SuvH39H1-siRNAs and (d) Dnmt1-siRNA was determined by quantifying its gene transcripts. NG and HG = cells in 5 mM or 20 mM D-glucose respectively, HG/Suv-si and HG/Dnmt1-si = cells transfected with Suv39H1-siRNA or Dnmt1-siRNA, and incubated in 20 mM D-glucose, Suv-si and Dnmt-si = cells transfected with Suv39H1-siRNA or Dnmt1-siRNA. *p < 0.05 vs NG and ^#p < 0.05 vs HG.