Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jul 8;11:14176. doi: 10.1038/s41598-021-93584-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The interaction between HBx and Pin1. Cell lysate of BL21 transfected with Flag-tagged HBx expression plasmids was incubated with Ni2 + -NTA beads adding or not adding purified His₆-tagged Pin1. HBx was detected in both the whole cell lysate isolated from Ni²⁺-NTA beads and imidazole eluent (A). Co-immunoprecipitation assay showed that with the transfection of Flag-tagged HBx, endogenous Pin1 protein pulled down by HBx was detected in eluent after incubated with anti-Flag beads, while HBx was detected in eluent after incubated with anti-Pin1 beads (B). Immunofluorescence microscopy shows the common position of Pin1 and HBx in both cytoplasm and nucleus (C).