Fig. 4. Effect of ZFP36 and CREBBP deficiency on ischemia–reperfusion (I/R)-induced lung damage and inflammation in vivo.

A CREBBP mRNA and protein expression in lung tissues of mice transfected with shZFP36 or empty vector (scr) were analyzed by RT-qPCR (n = 6 per group, **P < 0.01) and Western blot. B–D Arterial blood PaO₂ (B), lung water content (C), and the protein content of BALF (D) were measured. E–G Levels of IL-1β (E), TNF-α (F), and IL-6 (G) were measured by ELISA. H H&E staining of lung sections, scale bar: 50 μm.