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. 2021 Jul 8;12(7):685. doi: 10.1038/s41419-021-03950-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — C57BL/6 mice were subjected to 60 min intestine ischemia followed by 60 min reperfusion, and then detections were performed at 1, 3, 7, and 14 days. Sham mice were included as a control. A–D ZFP36 (A), TGF-β1 (B), COL1A1 (C), and COL3A1 (D) mRNA expression in lung tissues of each group were analyzed by RT-qPCR (n = 6 per group, **P < 0.01) and Western blot. E ZFP36, TGF-β1, COL1A1, and COL3A1 protein expression in lung tissues of each group by Western blotting. F Immunohistochemical staining of lung tissues of mice with stable knockdown of ZFP36 or scr for sham and I/R after 14 days. Scale bars: 50 μm. G, H H&E stain (G) and Masson trichrome (H)-stained lung sections. Scale bar: 50 μm.