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. 2021 Jul 8;11:14091. doi: 10.1038/s41598-021-93512-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Mesoderm specific defects in twi::Gal4 > UAS—htl-CRY2-mCherry embryos kept under constant light. (A) Tinman staining pattern in illuminated wild-type (WT) embryos, twi::Gal4 > Opto-htl embryos kept under dark and illuminated conditions. (B) twi::Gal4 > Opto-htl embryos stained with Fas3 and imaged at stage 16 under dark conditions (top) and when illuminated by constant 488 nm light (bottom). Svp-positive cells, marked with an asterisk, are identified by lower Fas3 expression. Arrows denote branching of the heart structure. (C) twi::Gal4 > Opto-htl embryos stained with Mef-2 and Eve antibodies at stage 16 under dark and illumination conditions. Arrows correspond to phenotypes described in the text. (D) Muscle structure in twi::Gal4 > Opto-htl embryos kept under dark and light conditions visualised using Phalloidin staining. (E) As (D) with muscles imaged at higher magnification. Arrows point to extended Ventral Oblique muscles, brackets represent extended muscle fibres, square box shows unfused myoblasts. Scale bar = 50 μm unless stated otherwise. VM = Visceral mesoderm, CB = Cardioblasts, PC = Pericardial cells.