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. 2021 Jul 8;11:14091. doi: 10.1038/s41598-021-93512-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Opto-htl induced phenotype depends on light intensity. (A) Heart at stage 16 in a hand::GFP control embryo. (B) Heart at stage 16 in a twi::Gal4 > hand::GFP;Opto-htl embryo kept in the dark throughout development. (C) Representative images of the heart at stage 16 in twi::Gal4 > hand::GFP;Opto-htl embryos illuminated from stage 5 till stage 16, at different light intensities. (D) Quantification of hand:GFP cell number for embryos illuminated at different intensities (n = 5 for each condition). The top and bottom of the box represent the third quartile and the first quartile, respectively. The median divides the box. The whiskers are error bars: upward from the third quartile to the maximum, and downward from the first quartile to the minimum. (E) Survival rate of twi::Gal4 > Opto-htl embryos when illuminated at different intensities throughout development. Scale bar = 50 μm.