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. 2021 Jul 8;11:14091. doi: 10.1038/s41598-021-93512-1

Table 2.

Hatching rate of twi::Gal4 > Opto-htl embryos illuminated from specified stages until end of embryogenesis.

Illumination onwards stage x till hatching (stage x =) Illumination time from cellularization stage Stage Hallmark No. of embryos screened No. of embryos hatched % hatchability
5 0 min Cellularisation 31 2 6.4
6/7 40 min Gastrulation 7 0 0
8/9 1–2:10 h Germ band Elongation starts 5 0 0
10 2:10 h Germ band Elongation finishes 40 1 2.5
12 5:10 h Germ Band retraction starts 34 0 0
13 7:10 h Midgut primordium forms a sack 19 17 89
14 8:10 h Head Involution and DC starts 11 11 100
15 9:10 h Dorsal Closure 18 18 100
16 11 h Gut constrictions appear 12 11 91.6
17 14 h First movements start; Trachea filling 16 16 100