During IVM, low oxygen in combination with low glucose significantly reduces subsequent development to blastocyst, which is rescued by increasing glucose concentration. Blastocyst rate (% from starting number of COCs) of embryos developed from COCs matured in two different oxygen concentrations, and two different glucose concentrations (Experiment 1, 2×2 factorial design). Data presented as mean ± SEM of (n = 5 independent replicates, 40±5 COCs/treatment group/replicate). Means with different superscripts indicate significant differences between treatment groups (P < 0.05)