Fig. 5.

Development to the blastocyst stage is reduced when developed from COCs matured under low oxygen with low glucose, a low volume of medium per COC and a high density of COCs per drop. Blastocyst rate (% from starting number of COCs) of embryos developed from COCs matured in two different oxygen concentrations, with 3 differing treatment parameters (Experiment 4: glucose concentration; volume of medium/COC; number of COCs/drop). Data presented as mean ± SEM of % (n = 5 independent replicates with 40±5 COCs/treatment group/replicate). Superscripts indicate significant differences between treatment groups (P < 0.05)