Table 1.

Experimental design. Bold entries indicate parameters altered within an experiment (n = 5 independent replicates per experiment, with 40±5 COCs per treatment per replicate)

Experiment	Treatment group	Oxygen concentration (%)	Glucose (mM)	Volume of drop (ul)	Number of COCs per drop	Volume of available medium
1	1	20	2.3	400	40	10 μl/COC
	2	20	5.6	400	40	10 μl/COC
	3	7	2.3	400	40	10 μl/COC
	4	7	5.6	400	40	10 μl/COC
2	1	20	2.3	400	40	10 μl/COC
	2	20	2.3	800	40	20 μl/COC
	3	7	2.3	400	40	10 μl/COC
	4	7	2.3	800	40	20 μl/COC
3	1	20	2.3	400	40	10 μl/COC
	2	20	2.3	100	10	10 μl/COC
	3	7	2.3	400	40	10 μl/COC
	4	7	2.3	100	10	10 μl/COC
4	1	20	2.3	400	40	10 μl/COC
	2	20	5.6	200	10	20 μl/COC
	3	7	2.3	400	40	10 μl/COC
	4	7	5.6	200	10	20 μl/COC