Fig. 1.

Expression of AURKB and AURKC by Western blot. Expression of AURKB and AURKC examined by Western blot analysis of CML (JURL-MK1, K562 and MEG-01) and AML (KG-1a, NB4 and NOMO-1) cell lines cultured in suspension and human gonadal tissues (ovarian cortex and testicular tissue). GAPDH was used as a loading control. AURKB was detectable in all CML/AML cell lines with as a single protein band of approximately 35 kDa (NB4 and NOMO-1), or in combination with a slightly smaller protein band (JURL-MK1, K562, MEG-01 an KG-1a). AURKB was undetectable in ovarian cortex tissue or testicular tissue. AURKC was detected in JURL-MK1, K562, MEG-01, KG-1a, ovarian cortex tissue and testicular tissue at different expression levels but absent in NB4 and NOMO-1