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. 2021 Mar 16;38(6):1571–1588. doi: 10.1007/s10815-021-02081-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Morphology and viability of follicles. Ovarian cortex tissue was exposed to a 24-h ex vivo treatment of solvent-only or 1 μM GSK1070916, washed and cultured for an additional 24 h, to allow tissue damage to become apparent. At least 100 follicles were evaluated per patient per condition. a Graphical depiction of the percentage of intact and damaged follicles after treatment. Morphology was examined by HE staining. No statistically significant difference (P = 0.83) was observed between follicles from the control or the tissue treated with the inhibitor. b Graphical depiction of the percentage of viable Neutral red positive follicles and non-viable Neutral red negative follicles. Ovarian cortex tissue was examined by Neutral red staining. No statistically significant difference (P = 0.92) was observed between follicles from the control or the tissue treated with the inhibitor