Fig. 4.

Sperm lacking Atp1a4 does not properly respond to hypoosmotic shock. a Sperm from WT and Atp1a4 KO mice was collected from the indicated tissue sources and subjected to a medium with an osmolarity of 53 mOsmol/kg for 60 min. Sperm swelling was analyzed morphologically under phase contrast microscopy. The number of cells presenting signs of swelling were quantified and expressed as percent of the total cells in the sample. Values represent the mean + SEM of 3 different experiments. Asterisks indicate statistical differences, with P < 0.05. b Representative pictures of caudal epididymal sperm from WT and Atp1a4 KO mice subjected to the hypoosmotic test