Fig. 6.

Detergent treatment partially recovers flagellar angulation of sperm from Atp1a4 KO mice. Caudal epididymal sperm from Atp1a4 KO mice was collected in medium with an osmolarity of 250 mOsmol/kg and were incubated in the absence and presence of 0.1% of Triton-X100 for 10 min. Then, the number of sperm with a straight or bent flagellum was quantified, identifying angulation from total retroflexion. Cells were scored and expressed as percent of the total cells in the sample. Values represent the mean + SEM of 3 different experiments. Asterisks show statistically significant differences, with P < 0.05