Fig. 7.

Proteomic analysis of structural proteins in sperm from WT and Atp1a4 KO mice. Caudal epididymal sperm from Atp1a4 KO mice was collected using Percoll separation. Samples were subjected to 2D-gel electrophoresis for quantification and Maldi-TOF/TOF for identification. a Gene classification obtained from proteome analysis with cutoff values of 1.4-fold and P < 0.1. Proteins were functionally categorized based on the PANTHER GO Molecular Function. Listed proteins correspond to the “structural molecular activity” category. b Immunoblot analysis of selected structural proteins (ODF2, DNAI, and GPx4). c Relative protein levels as determined by densitometry from the immunoblot analysis. Protein levels were normalized using tubulin as a control and expressed relative to the level of the corresponding protein from WT sperm. Values represent the mean + SEM of 3 different experiments. Asterisks show statistically significant differences, with P < 0.001