Fig. 4.
Intracellular free calcium ([Ca2+]i) oscillation patterns after parthenogenetic activation of oocytes treated with the endoplasmic reticulum stress inducer tunicamycin (TM). a, b Oocytes were treated with tunicamycin (TM) for 1.5 h. The 0-μM group was the control group, and 2.5, 5, 10, 20, 30, and 50 μg/ml TM were respectively added to the M2 medium. c [Ca2+]i oscillation parameters in oocytes treated with gradient TM concentrations ranging from 0 to 50 μg/ml. d The pronucleus (PN) rate of oocytes with ER stress induced by TM. N = 25 to 30 for each group. Data represent mean ± SD, and chi-square test and one-way ANOVA are used for statistical analysis. *P < 0.05 versus the control group