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Immunostaining for p16. (a) Oral tongue squamous cell carcinoma with keratinization (hematoxylin and eosin (HE) ×100); (b) lack of p16 expression in tumor cells (immunohistochemistry ×100). (c) Regional lymph node metastasis of unknown primary, with non-keratinizing squamous cell carcinoma (HE ×100); (d) expression of p16 in tumor cells indicated by diffuse nuclear and strong cytoplasmic staining (immunohistochemistry ×100).
