FIGURE 4.

IIIM-941 inhibited NLRP3 inflammasome by inducing autophagy in J774A.1 and BMDM cells (A) IIIM-941 at 5 and 10 µM increased the expression of pAMPK (Thr-172) and induced autophagy as represented by increased levels of LC3-II in a time dependent manner in J774A.1 cells as analyzed by western blotting (B) Western blotting anlayis of autophagy and NLRP3 inflammasome proteins was done under the conditions used for induction of NLRP3 inflammasome in J774A.1 cells and (C) in BMDMs. For induction of NLRP3 inflammasome, similar conditions were used as in Figure 1A. The blots presented here are representative in nature. ImageJ software was used to quantify the band density of the western blots. The quantified data of three independent experiments ±SD for Figure B and C is presented in the Supplementary Figures S3A,B. The quantified data presented here for Fig A is from three independent experiments ±SD. p value < 0.05 was considered significant, ***p < 0.001, **p < 0.01, *p < 0.05.