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Schematic diagram of animal operation. A 150 µL mixture of 30% DMSO and 70% PBS with 3.0 mg/kg body weight of MNU was injected into the anterior chamber of rabbits through the corneal limbus with a sterile 30-gauge steel needle. Within 1 week before and after injection of MNU, rabbit eyes were irrigated with HRS 3 times a day for 3 minutes and 3 drops per second, whereas the control eyes were irrigated with saline (n = 8).
