Table 1. Antibodies used in immunohistochemistry.

Antigen	Host species	Clonality	Dilution	Antigen retrieval	Manufacture
Cytokeratin AE1/AE3	Mouse	Monoclonal	Undiluted	Autoclaveda	Dako (Glostrup, Denmark)
CK5	Rabbit	Monoclonal	1:400	Microwavedb	Abcam Inc. (Tokyo, Japan)
α-SMA	Mouse	Monoclonal	1:500	Microwavedb	Dako
β-catenin	Rabbit	Polyclonal	1:250	Microwavedc	Dako
PCNA	Rabbit	Polyclonal	1:200	n.a.	Dako

α-SMA, α-smooth muscle actin; CK5, cytokeratin 5; n.a., not applicable; PCNA, proliferating cell nuclear antigen. ^a At 121°C for 10 min in 10 mM citrate buffer (pH 6.0), ^b at 90°C for 10 min in Dako target retrieval solution (pH 9.0), ^c at 90°C for 10 min in 10 mM citrate buffer (pH 6.0).