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. 2021 Jun;9(12):1005. doi: 10.21037/atm-21-2632

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2021 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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TCONS_00034812 positively regulate the expression of miR-21 in HAOSMCs through methylation. HAOSMCs were transfection with TCONS_00034812 expression vector, TCONS_00034812 siRNA or miR-21 mimic to analyze the interaction between them. Transfections were completed at 48 h after the transfection by proceeding RT-qPCR (A). The effects of overexpression of TCONS_00034812 (B) and silencing of TCONS_00034812 (C) on miR-21 expression and miR-21 gene methylation were evaluated by RT-qPCR and MSP. The effects of miR-21 overexpression on TCONS_00034812 were estimated by RT-qPCR (D). *, P<0.05. HAOSMCs, human aortic smooth muscle cells; C, untreated control; NC, miRNA control; NC siRNA, small interfering RNA control.