Fig. 1.

Generation of an AlbR line and BZ compound screens: A) Time in culture and induction of AlbR in a WB-1B line starting at 0.1 μM and finishing at 0.5 μM over 400 days. B) IC₅₀ dose response curves for albendazole, podophyllotoxin and metronidazole in AlbS and AlbR isogenic lines, demonstrating a substantial shift in IC₅₀ in the AlbR for albendazole and podophyllotoxin but not for metronidazole. C) Bar chart of IC₅₀ values for AlbS and AlbR isogenic lines (left axis) as well as the calculated RF for the AlbR (right axis) for BZ compounds. Compounds are listed from left to right in order of potency. The full dose response curves of all BZ compounds are in Supplementary Fig. S1. Thiabendazole, albendazole sulfone and reduced mebendazole were not sufficiently active in both lines at 10 μM to calculate an IC₅₀, and therefore has not been shown.