Skip to main content
. 2021 Mar 25;12(2):689–713. doi: 10.1016/j.jcmgh.2021.03.008

Table 6.

PCR Cycling Conditions for DNA Library Amplification

Cycle step Temperature Time Cycles
Initial denaturation 98ºC 30 seconds 1
Denaturation 98ºC 10 seconds 8a
Annealing/extension 65ºC 75 seconds
Final extension 65ºC 5 seconds 1
Hold 4ºC ∞
a

Optimized for 100 ng input DNA.