Figure 7.

DEGs between auCC and aRCC colonic mucosa. (A, B) Normalized log₂-transformed fold changes (using the regularized log function in R) of RNA-seq transcript counts (log TC) from DEGs between auCC and aRCC CC mucosa (A) displayed as a heatmap or (B) as detailed individual plots including samples from Hc subjects and all CC groups (median with interquartile range). Selected genes for further RT-qPCR validation are highlighted in red. (C) Log₂ fold changes (–ΔCt values) (median with interquartile range) in gene expression of B3GNT8, DENN4A, and IFIT3 analyzed by quantitative PCR. HPRT1 was used as a housekeeping control. Hc subjects are shown in green, auCC samples in orange, itCC samples in blue, and aRCC samples in purple. n = 9–13 samples per group for RNA-seq analyses; n = 13–20 samples per group for RT-qPCR validation. Statistically significant differences relative to Hc samples are shown as ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001, unless other comparison is indicated; statistically significant differences relative to itCC samples are shown as #P < .05, and ##P < .01.