| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-mouse PD1 (anti-CD279) | Bio-X-Cell | Clone (RMP1-14); RRID: AB_10949053 |
| Rat IgG2a isotype control antibody | Bio-X-Cell | 2A3; RRID: AB_1107769 |
| Anti-mCD4 for depletions | Bio-X-Cell | Clone GK1.5; RRID: AB_1107636 |
| FITC-anti-mCD4 for surface staining | BioLegend | Clone GK1.5; RRID: AB_312691 |
| APC-anti-CD4 for intracellular staining | BioLegend | Clone GK1.5; RRID: AB_312697 |
| Anti-mCD8 for depletions | Bio-X-Cell | Clone YTS169.4; RRID: AB_10950145 |
| PE-CY7-anti-mCD8a for staining | BioLegend | Clone 53.6-7; RRID: AB_312761 |
| Anti-Phytopthora Ig AFRC MAC 51 control antibody for depletions | In-house (L.Boon) | From hybridoma: https://www.sigmaaldrich.com/US/en/product/sigma/cb_85060405 |
| PE-anti-CD49b | BioLegend | Clone DX5; RRID: AB_313414 |
| Violet 421-anti-CD3e | BioLegend | Clone 145-2C11; RRID: AB_11203705 |
| PerCP/Cy5.5-anti-B220 | BioLegend | Clone RA3-6B2; RRID: AB_893356 |
| APC-anti-CD44 | BioLegend | Clone IM7; RRID: AB_312963 |
| PE-anti-LAG3 | BioLegend | Clone C9B7W; RRID: AB_2133343 |
| APC-anti-Tim3 | BioLegend | Clone RMT3-23; RRID: AB_2561656 |
| PE-anti-CTLA4 | BioLegend | Clone UC10-4B9; RRID: AB_313255 |
| APC-anti-PD1 | BioLegend | Clone 29F.1A12; RRID: AB_2159183 |
| PE-Cy7-anti-F4/80 | BioLegend | Clone BM8; RRID: AB_893478 |
| Violet 421-anti-CD11b | BioLegend | Clone M1/70; RRID: AB_2562904 |
| PerCP-anti-CD11b | BioLegend | Clone M1/70; RRID: AB_2129375 |
| FITC-anti-Foxp3 | eBiosciences | Clone FJK-16s; RRID: AB_465243 |
| PE-anti-CD25 | BD Sciences | Clone 7D4; RRID: AB_1645250 |
| PE-anti-PDL1 | BioLegend | Clone 10F.9G2; RRID: AB_2073556 |
| APC-anti-MHCII | BioLegend | Clone M5/114.15.2; RRID: AB_313329 |
| PE-anti-H2-Kb | eBioscience | Clone AF6-88.5.5.3; RRID: AB_10598797 |
| PE-anti-H2-Kd | eBioscience | Clone SF1-1.1.1; RRID: AB_2043875 |
| APC-anti-H2-Db | BioLegend | Clone KH95; RRID: AB_2565862 |
| FITC-anti-H2-Ld | Invitrogen | Clone 30-5-7S; RRID: AB_2539390 |
| Bacterial and virus strains | ||
| Oncolytic herpes simplex virus | Sorrento Therapeutics (San Diego, CA) | HSV1716 |
| Chemicals, peptides, and Recombinant proteins | ||
| RPMI 1640 | Thermo Fisher Scientific | A1049101 |
| DMEM | Thermo Fisher Scientific | 12430112 |
| Fetal bovine serum (FBS) | Thermo Fisher Scientific | 26,140 |
| ACK red cell lysis buffer | Lonza | 10-548e |
| Mouse Fc blocking reagent | BD Biosciences | Clone 2.4G2; RRID: AB_394656 |
| Streptavidin-APC | Molecular Probes | SA1005 |
| Biotinylated H2-Ld mouse MuLV gp70 SPSYVYHQF | NIH Tetramer Core Facility (NTCF) | AH1 GP70 peptide |
| Biotinylated H2-Kb mouse MuLV gp70 KSPWFTTL | NIH Tetramer Core Facility (NTCF) | p15E GP70 peptide |
| Critical Commercial assays | ||
| SureSelectXT mouse all exon library kit | Agilent Technologies | G7550 |
| TruSeq Stranded total RNA library kit | Illumina, Inc. | 20020596 |
| MycoAlert mycoplasma detection kit | Lonza | LT07-318 |
| Cell titer 96 Aqueous non-Radioactive cell Proliferation assay | Promega | G5421 |
| RNeasy plus Mini kit | Qiagen | 74,134 |
| SuperScript II reverse Transcriptase | Life Technologies | 18064022 |
| iTaq universal SYBR green supermix kit | Bio-Rad | 1725120 |
| Cell fixation and permeabilization kit | Invitrogen | GAS001S100 and GAS002S100 |
| Experimental models: Cell lines | ||
| F420 osteosarcoma cell line | Kind gift from Jason Yustein | Cells were derived from genetically engineered mouse model reported in Zhao et al. (2015) |
| K7M2 osteosarcoma cell line | ATCC | ATCC Cat# CRL-2836, RRID:CVCL_V455 |
| Experimental models: Organisms | ||
| C57BL/6 female mice | Envigo | C57BL/6NHsd |
| BALB/c female mice | Envigo | Balb/cAnNHsd |
| Deposited data | ||
| Raw and analyzed RNA-seq data | This paper | Gene expression omnibus: GSE166282 |
| Raw tumor exome sequencing data | This paper | Sequence read archive: PRJNA698961 |
| RNA-seq data | This paper | Mendeley: https://doi.org/10.17632/cgswh7x24z.1 |
| Software and algorithms | ||
| bcl2fastq conversion software (version 2.20.0.422) | Illumina, Inc. | https://support.illumina.com/sequencing/sequencing_software/bcl2fastq-conversion-software/downloads.html |
| HaplotypeCaller | Genome Analysis Toolkit (GATK) | https://gatk.broadinstitute.org/hc/en-us/articles/360037225632-HaplotypeCaller |
| Mutect2 | GATK | https://gatk.broadinstitute.org/hc/en-us/articles/360037593851-Mutect2 |
| CHURCHILL | Kelly et al., (2015) | Email lead contact for more information |
| pVACseq | Hundal et al., (2016) | https://github.com/griffithlab/pVACtools |
| Salmon (version 0.9.1) | (Patro et al., 2017) | https://github.com/COMBINE-lab/salmon |
| Mus musculus reference genome | National Center for Biotechnology Information RefSeq | GRCm38.p4_rna.fna |
| Mus musculus endogenous retroviral reference sequences | GEVE | Mmus38.geve.nt_v1.fa |
| gp70 sequence | GenBank | DQ359272.1 |
| DAVID (version 6.8) | (Dennis et al., 2003) | https://david.ncifcrf.gov |
| CIBERSORT | (Newman et al., 2015) | https://cibersort.stanford.edu |
| DESeq2 (version 1.26.0) | (Love et al., 2014) | http://bioconductor.org/packages/release/bioc/html/DESeq2.html |
| GraphPad Prism version 7.0a | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| FlowJo version 10.0.3 | Tree Star | https://www.flowjo.com/ |
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