Figure 3.

RIG-I and MDA5 recognizes viral RNA fragment of SARS-CoV-2. (A–I) The viral RNA fragment containing 24001–25000 (A–F) or 24001–24200 region (G–I) was transfected into WT, RIG-I KO, MDA5 KO, and Riplet KO HEK293 cells, and then the expression of the cytokines was determined by RT-qPCR. The data represent the mean ± SD (n = 3, *p < 0.05, t-test). The data shown is representative of at least two independent experiments. (J) The viral RNA fragment containing 24001–24200 region was transfected into HeLa, A549, and THP-1 cells, and then expression of IFN-β was determined by RT-qPCR. The data represent the mean ± SD (n = 3, *p < 0.05, t-test). The data shown in representative of at least two independent experiments.